中英文说明书丨Jackson正常兔血清

【中英文说明书丨Jackson正常兔血清】动物实验是医学科学发展的不可缺少学科 。 家兔又是动物实验时的shou选动物 , 为使动物实验资料和科学性更加完善 , 接下来一起了解下艾美捷Jackson正常兔血清 。
艾美捷Jackson正常兔血清产品可应用于大多数免疫学实验用作封闭液或空白对照.
艾美捷Jackson 正常兔血清使用:
正常兔血清是以冻干粉形式提供的 , 本品需要先溶解配制成100%原液后再稀释使用 。 溶解方法:用5 mL蒸馏水(dH2O)充分溶解冻干粉(如果不澄清 , 可对其离心处理) , 此时得到的即是100%原液 。 然后按照1:20的比例用缓冲液 , 如PBS或PBST , 稀释到需要的5%工作液 。 也可以根据自身实验条件 , 稀释到合适的工作液浓度 。
艾美捷011-000-120正常兔血清化学性质:
全名:正常兔血清丨Normal Rabbit Serum
Cat#:011-000-120
规格:5ml
蛋白浓度(Protein concentration):60 mg/mL
缓冲液(Buffer):0.01 M磷酸钠 , 0.25 M氯化钠 , pH 7.6
防腐剂(Preservative):0.05%die氮化钠
运输与保存方法:冰袋运输 。 冻干粉保存于2-8℃ 。
Information
Normal serums are lipid extracted to improve clarity dialyzed against phosphate buffered saline (PBS) containing sodium azide and freeze dried. Normal serum diluted to 5% (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific conserved sequence and/or Fc receptor binding. Best results are obtained with diluted normal serum from the same host as the labeled antibody as a separate incubation step before addition of the primary antibody.
Usage
Physical State: Freeze-dried solid
Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Preservative: 0.05% Sodium Azide
Suggested Working Concentration or Dilution Range:
For blocking purposes use 5% (v/v) solution (1:20 dilution from rehydrated volume). Rehydration according to instruction yields 100% serum.
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