皮肤|?不可思议！只需25步，用一块芯片就能把皮肤变成血管、神经细胞 tissue|组织|论文|神经细胞|纳米

<br /> 文章图片 <br /> <br /> nanotransfection 示意图 | 来源：印第安纳大学 <br /> 作者称，这种「组织纳米转染技术」（tissue nanotransfection, TNT）所需要的设备是可以标准化批量生产的。「组织纳米转染技术」的最大优点是不需要用病毒作为载体，从而最大限度地降低了炎症反应和细胞死亡的风险。<br /> 发明这项技术的印第安纳再生医学和工程中心（Indiana Center For Regenerative Medicine And Engineering）主任 Chanda Sen 表示：“这篇论文将让更多人一同参与到再生医学中来。 ”<br /> 制备空心微针阵列 <br /> 根据「纳米转染」说明书，改变组织功能共分为三个步骤（共 25 个具体操作环节）：制备空心微针阵列、制备 TNT 器件，以及对细胞进行转染。首先要考虑的是使用哪种微针阵列，各种不同形态的微针总有一款适合您。对于在局部皮肤导入质粒 DNA ，作者建议使用尖端扁平的微针（见图 1A）。对于深层组织的导入，作者建议使用尖端锋利的微针以促进组织穿透( 见图 1B 或 1C) 。<br /> <img alt="皮肤|?不可思议！只需25步，用一块芯片就能把皮肤变成血管、神经细胞" onload="javascript:ImgReSize(this)" src="https://p5.itc.cn/q_70/images03/20220102/c198aa397bd04161979395e10dd2da2e.png" /> <br /> 文章图片 <br /> <br /> 各种微针 | 来源：论文 <br /> 选好款式以后我们就要进行手工制作了。<br /> 首先需要在双面抛光的 4 英寸硅（Si100）晶片上覆盖一层光刻胶，然后使用博世（Bosch）工艺对晶片进行深反应离子刻蚀（DRIE）。根据转染需要可以制备三种不同的硅空心微针，包括顶端扁平的一型（图 1E ， Type I），顶端突出的二型（Type II）或带有偏心孔的三型（Type III）（图 1F ， g) 。后两种尖端锋利的微针阵列更有助于穿透到组织当中。</p> <div class="dede_pages"><ul><li><a 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